Protocols and reference information for a large number of cell lines are available in neon® cell database Using the neon® transfection system 10 μl kit (thermo fisher scientific), 1 to 5 × 10 5 ncms or um cells (mel185 cell line (wt for gnaq/gna11) ; Rnp delivery is accomplished using the thermo fisher neontm transfection system.
Glowing neon line database icon isolated on brick Vector Image
A reference for electroporation settings for a wide variety of cell types is included.
Substantial findings have emerged from studies investigating the implications of neon electroporation.
Efficiently transfect mammalian cells including primary and immortalized hematopoietic cells, stem cells, and primary cells. Our crispr neon electroporation protocol was designed to allow highly efficient transfection of rnp complexes into standard cell lines. Learn about the flexability, simplicity and versatility of the neon®. Achieve high editing efficiencies in your crispr.
However, these conditions may have to be modified slightly for your particular cell line, since. Key results indicated that specific cell lines, such as hek293, can exhibit up. Search the transfection protocols and citations database for optimized electroporation protocols on different cell lines.
